Journal: Journal of Translational Medicine

Article Title: Single-cell transcriptomic landscape of the mid-secretory eutopic endometrium reveals receptivity defects in adenomyosis

doi: 10.1186/s12967-026-07866-z

Figure Lengend Snippet: Identification, functional characterization, and trajectory analysis of stromal cell subclusters in adenomyosis (AM). ( A ) UMAP visualization of stromal subclusters (Str1–Str4). ( B ) Dot plots depicting the average expression of established markers indicated ecotype. ( C ) Pseudotime trajectory showing the progression of Str1, Str2, Str3 and Str4. ( D ) Gene Ontology (GO) terms significantly enriched across gene clusters with distinct pseudo-temporal patterns. ( E ) The distribution of DIO2 , PGR , and WNT5A in stromal cell subclusters by UMAP. ( F ) Violin plot of inflammatory scores across stromal subclusters. ( G ) Box plots of the relative proportions of AM and control cells in each stromal subcluster. ( H ) Separate pseudotime trajectories of stromal subclusters in control and AM groups

Article Snippet: Control, LGR5, SOX9, and DIO2 overexpression plasmids (GeneCopoeia, China) were introduced using X-tremeGENE 9 (Roche, USA).

Techniques: Functional Assay, Expressing, Control

Journal: Journal of Translational Medicine

Article Title: Single-cell transcriptomic landscape of the mid-secretory eutopic endometrium reveals receptivity defects in adenomyosis

doi: 10.1186/s12967-026-07866-z

Figure Lengend Snippet: DIO2 ⁺ Str4 stromal cells exhibit SASP-like activity and drive decidualization. ( A , B ) Representative immunohistochemistry (IHC) images of DIO2, CXCL12, IGFBP3, and MMP14 in mid-secretory eutopic endometrial tissues from controls and adenomyosis (AM) patients ( n = 12). Scale bars, 50 μm. ( C ) ELISA quantification of decidualization markers IGFBP1 and PRL in control and AM tissues ( n = 12). ( D ) Senescence-associated β-galactosidase (SA-β-gal) staining in control HESCs undergoing in vitro decidualization. ( E ) RT-qPCR analysis of SASP-related cytokines CXCL14, TIMP3, and IL15 in decidualized control HESCs ( n = 3). ( F ) ELISA measurement of secreted SASP cytokines in culture supernatant ( n = 3). ( G – K ) Effects of DIO2 knockdown on β-gal activity ( G ), SASP cytokine mRNA expression ( H ), and secreted protein levels ( I – K ) in control HESCs ( n = 3). ( L , M ) F-actin filaments staining in control HESCs following DIO2 knockdown during in vitro decidualization, visualized with Alexa Fluor 555–conjugated phalloidin. Scale bar = 50 μm, n = 3. Data are presented as the mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: Control, LGR5, SOX9, and DIO2 overexpression plasmids (GeneCopoeia, China) were introduced using X-tremeGENE 9 (Roche, USA).

Techniques: Activity Assay, Immunohistochemistry, Enzyme-linked Immunosorbent Assay, Control, Staining, In Vitro, Quantitative RT-PCR, Knockdown, Expressing

Journal: Nutrients

Article Title: Iodoquinoline-Biofortified Lettuce as a Safe and Bioavailable Dietary Iodine Source: In Vivo Study in Rats

doi: 10.3390/nu18010036

Figure Lengend Snippet: Relative expression of ( A ) Dio1 , ( B ) Dio2 , ( C ) Slc5a5 (NIS), and ( D ) Tpo genes in the thyroid gland of experimental rats. Results are presented as mean ± standard deviation (n = 7). Bars marked with different letters differ significantly at p ≤ 0.05. Description of experimental groups: Group #1 —rats fed the AIN-93G (control) diet; Group #2 —rats fed the AIN-93G diet with the addition of lyophilized non-biofortified lettuce, with KI from the mineral mixture providing iodine in the amount recommended for this diet; Group #3 , #4 , #5 —rats fed the AIN-93G diet with the addition of lyophilized iodine-biofortified lettuce in the form of potassium iodate (Group #3), 8-hydroxy-7-iodo-5-quinolinesulfonic acid (Group #4), and 5,7-diiodo-8-quinolinol (Group #5), providing iodine in the amount recommended for this diet; Group #6 , #7 , #8 —rats fed the AIN-93G diet with the addition of lyophilized iodine-biofortified lettuce in the form of potassium iodate (Group #6), 8-hydroxy-7-iodo-5-quinolinesulfonic acid (Group #7), and 5,7-diiodo-8-quinolinol (Group #8), providing iodine in an amount twice that recommended for this diet.

Article Snippet: Quantitative real-time PCR (qPCR) was performed to assess gene expression levels using TaqMan ® Gene Expression Assays (Thermo Fisher Scientific) specific for the following rat genes: Dio1 (Rn00572183_m1), Dio2 (Rn00581867_m1), Slc5a5 (Rn00583900_m1), and Tpo (Rn00571159_m1).

Techniques: Expressing, Standard Deviation, Control